Original Research

The impact of extract of mistletoe on tumour cells and hemostasis of human plasma

Rostyslav Y. Marunych, Denis L. Kolesnik, Olga N. Pyaskovskaya, Galina I. Solyanik, Andrew V. Rebriev, Tamara M. Chernyshenko, Natalia E. Lugovska, Tetiana V. Kozytska, Andriy V. Gudzenko
Journal of Medicinal Plants for Economic Development | Vol 7, No 1 | a186 | DOI: https://doi.org/10.4102/jomped.v7i1.186 | © 2023 Rostyslav Y. Marunych, Denis L. Kolesnik, Olga N. Pyaskovskaya, Galina I. Solyanik, Andrew V. Rebriev, Tamara M. Chernyshenko, Natalia E. Lugovska, Tetiana V. Kozytska, Andriy V. Gudzenko | This work is licensed under CC Attribution 4.0
Submitted: 15 November 2022 | Published: 25 May 2023

About the author(s)

Rostyslav Y. Marunych, Department of Structure and Function of Proteins, Palladin Institute of Biochemistry, the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Denis L. Kolesnik, Laboratory of Molecular and Cellular Mechanisms of Metastasis, RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Olga N. Pyaskovskaya, Laboratory of Molecular and Cellular Mechanisms of Metastasis, RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Galina I. Solyanik, Laboratory of Molecular and Cellular Mechanisms of Metastasis, RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Andrew V. Rebriev, Department of Cell Signaling, Palladin Institute of Biochemistry, the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Tamara M. Chernyshenko, Department of Structure and Function of Proteins, Palladin Institute of Biochemistry, the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Natalia E. Lugovska, Department of Structure and Function of Proteins, Palladin Institute of Biochemistry, the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Tetiana V. Kozytska, Department of Histology and Embryology, Bogomolets National Medical University of Ukraine, Kyiv, Ukraine
Andriy V. Gudzenko, Department of Chemistry, PHEE, Kyiv Medical University, Kyiv, Ukraine

Abstract

Background: The plant Viscum album (Mistletoe) is a known source of biologically active substances, used in traditional medicine in Europe and Asia.

Aim: The goal was to study cytotoxic/cytostatic effect of mistletoe extract on tumour and normal cells and its influences on certain proteins of the blood coagulation system.

Setting: Mistletoe was collected in Ukraine in November 2020.

Methods: Water extract of V. album, both leaves and stems, was obtained and fractionated; it was characterised using high-performance liquid chromatography (HPLC) and mass spectrometry and tested on cancer cells and blood plasma.

Results: Extract demonstrated the presence of viscotoxins and carbohydrates and thermolabile compounds that enhance the activity of thrombin and factor X in plasma in the presence of calcium ions and increased the activated partial thromboplastin time (APTT) by 2.7 times. The cytotoxic/cytostatic action of the mistletoe fractions, the total extract and the fraction <10 000 Da were higher in relation to Lewis lung carcinoma (LLC) cells than with Vero cells. The IC50 of total extract for LLC cells was 35% (p < 0.05) lower than that for Vero cells (1.12 ± 0.2 and 1.71 ± 0.15 mg/ml). Thus, the IC50 of the fraction <10 000 Da for LLC cells was more than by 44% (p < 0.05) lower than that for Vero cells (2.07 ± 0.26 and 3.67 ± 0.41 mg/ml).

Conclusion: It was shown that both extracts exhibited a cytotoxic/cytostatic effect, more pronounced against tumour cells than normal cells and enhanced the clotting of blood plasma by thermolabile compounds by facilitating plasma coagulation.

Contribution: This research makes it possible to study mistletoe as a light and cheap anticancer therapy plant and cure blood coagulopathy or construct antibleeding bandages.


Keywords

V. album; plant extract; cancer cells; hemostasis; plasma coagulation; deficient plasma.

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