Original Research

Anti-motilities and anti-biofilm effects of Ageratum conyzoides L. methanol extract

Eli Compaoré, Moussa Compaoré, Vincent Ouédraogo, Ablassé Rouamba, Alimata Bancé, Mignini R. Dofini, Martin Kiendrebeogo
Journal of Medicinal Plants for Economic Development | Vol 9, No 1 | a270 | DOI: https://doi.org/10.4102/jomped.v9i1.270 | © 2025 Eli Compaoré, Moussa Compaoré, Vincent Ouédraogo, Ablassé Rouamba, Alimata Bancé, Mignini R. Dofini, Martin Kiendrebeogo | This work is licensed under CC Attribution 4.0
Submitted: 13 August 2024 | Published: 31 January 2025

About the author(s)

Eli Compaoré, Department of Biochemistry Microbiogy, Recherche/Sciences de la Vie et de la Terre, Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso
Moussa Compaoré, Department of Biochemistry Microbiogy, Recherche/Sciences de la Vie et de la Terre, Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso
Vincent Ouédraogo, Department of Biochemistry Microbiogy, Recherche/Sciences de la Vie et de la Terre, Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso
Ablassé Rouamba, Department of Biochemistry, Ecole Normale Supérieure, Ouagadougou, Burkina Faso
Alimata Bancé, Department of Traditional Medicine and Pharmacopoeia, Institut de Recherche en Sciences de la Santé, Ouagadougou, Burkina Faso
Mignini R. Dofini, Department of Traditional Medicine and Pharmacopoeia, Institut de Recherche en Sciences de la Santé, Ouagadougou, Burkina Faso
Martin Kiendrebeogo, Department of Biochemistry Microbiogy, Recherche/Sciences de la Vie et de la Terre, Université Joseph Ki-Zerbo, Ouagadougou, Burkina Faso

Abstract

Background: Infectious diseases are one of the leading causes of death worldwide because of antibiotic resistance. Ageratum conyzoides is one of the antimicrobial medicinal plants that is being used to fight various multi-resistant pathogenic bacteria in Burkina Faso.

Aim: The aim was to promote safe medicinal use of A. conyzoides by highlighting the anti-biofilm and anti-motility effects of its methanol extract.

Setting: The study was conducted at the Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso.

Methods: The antibacterial activities of methanol extract were evaluated by evaluating swimming, swarming and twitching motilities performed in an agar medium. The anti-biofilm effect was conducted in microtiter plates using the crystal violet method. The antioxidant and enzyme inhibition activities were evaluated using 2,2-diphényl-1-picrylhydrazyl; 2,2’-azino-bis (3-éthylbenzothiazoline-6-sulfonic acid), Ferric Reducing Antioxidant Power and conducting lipoxygenase test.

Results: From the study, 100 µg/mL and 200 µg/mL of extract presented significant inhibition of P. aeruginosa and E. coli swarming motility but did not exhibit a significant effect on P. aeruginosa swimming and E. coli twitching motilities. The extract was effective in reducing biofilm formation in a concentration-dependent manner without affecting bacterial growth. In addition, the extract showed some capabilities to inhibit lipoxygenase activity and exhibit antioxidant potential, which could contribute to the control of oxidative stress-related diseases.

Conclusion: From this study the anti-biofilm and anti-motility potential of the A. conyzoides extract provided the experimental background for the further development of antibacterial drugs.

Contribution: This study provided additional scientific evidence to support the use of A. conyzoides in traditional medicine against bacterial infections.


Keywords

Ageratum conyzoides; antioxidant; biofilm; motility; swimming; swarming, twitching; Pseudomonas aeruginosa; Escherichia coli

JEL Codes

I10: General; N37: Africa • Oceania; N57: Africa • Oceania

Sustainable Development Goal

Goal 3: Good health and well-being

Metrics

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