Original Research

Anticarcinogenic effect of alpha-lipoic acid and Jatropha gossypifolia leaves against cypermethrin-induced genotoxicity in rats

Opeyemi O. Faokunla, Oluseyi A. Akinloye, Regina N. Ugbaja, Abideen I. Adeogun
Journal of Medicinal Plants for Economic Development | Vol 2, No 1 | a45 | DOI: https://doi.org/10.4102/jomped.v2i1.45 | © 2018 Opeyemi O. Faokunla, Oluseyi A. Akinloye, Regina N. Ugbaja, Abideen I. Adeogun | This work is licensed under CC Attribution 4.0
Submitted: 16 November 2017 | Published: 26 February 2018

About the author(s)

Opeyemi O. Faokunla, Department of Biological Sciences, College of Natural and Applied Sciences, Wesley University, Nigeria
Oluseyi A. Akinloye, Department of Biochemistry, College of Biological Sciences, Federal University of Agriculture, Nigeria
Regina N. Ugbaja, Department of Biochemistry, College of Biological Sciences, Federal University of Agriculture, Nigeria
Abideen I. Adeogun, Department of Chemistry, College of Biological Sciences, Federal University of Agriculture, Nigeria


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Abstract

Background: Cypermethrin (Cyp), a synthetic pyrethroid developed for agricultural and public health applications, has become harmful to the environment and mammalian life owing to indiscriminate use.

Aim: This study aimed to investigate the protective effect of the methanolic extract of Jatropha gossypifolia leaves against Cyp-induced genotoxicity in rats using alpha-lipoic acid (α-LA) as standard drug.

Method: Fifty-six male Wistar rats, assigned to eight groups of seven animals each, were treated orally for 28 days as follows - Group 1: only corn oil (Control); Group 2: 20 mg/kg body weight (b.wt) of Cyp; Groups 3, 4 and 5: Cyp + 50 mg/kg b.wt of J. gossypifolia, Cyp + 100 mg/kg b.wt of J. gossypifolia and Cyp + 50 mg/kg b.wt of α-LA, respectively; Groups 6, 7 and 8: J. gossypifolia (50 mg/kg b.wt), J. gossypifolia (100 mg/kg b.wt) and α-LA (50 mg/kg b.wt), respectively. The P53 was assayed for using the plasma, while the micronucleus assay was carried out on the bone marrow.

Results: Results showed that exposure to Cyp significantly increased the number of micronucleated polychromatic erythrocytes (86.70%) as well as p53 concentration in both the liver and plasma (200.00% and 125.53%) compared to the control. Co-administration with methanolic extract of J. gossypifolia leaves significantly reversed these alterations in a dose-dependent manner.

Conclusion: The methanolic extract of J. gossypifolia leaves has the potential to ameliorate Cyp-induced genotoxicity in rats, and also, the toxicological evaluations of the plant extract and α-LA showed that it is relatively safe for use at the doses investigated.


Keywords

p53; cypermethrin; Jatropha gossypifolia; alpha-lipoic acid; genotoxicity

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