Original Research

Protective effects of neem (Azadirachta indica A. Juss) seed oil on carbon tetrachloride-induced hepatotoxicity in Wistar rats

MacDonald Idu, Oghale Ovuakporie-Uvo, Suzan O. Okojie
Journal of Medicinal Plants for Economic Development | Vol 1, No 1 | a6 | DOI: https://doi.org/10.4102/jomped.v1i1.6 | © 2017 MacDonald Idu, Oghale Ovuakporie-Uvo, Suzan O. Okojie | This work is licensed under CC Attribution 4.0
Submitted: 10 January 2017 | Published: 18 August 2017

About the author(s)

MacDonald Idu, Department of Plant Biology and Biotechnology, University of Benin, Nigeria
Oghale Ovuakporie-Uvo, Department of Plant Biology and Biotechnology, University of Benin, Nigeria
Suzan O. Okojie, Department of Plant Biology and Biotechnology, University of Benin, Nigeria


Background: Azadirachta indica (neem) seed oil was evaluated for its hepatoprotective effect. Liver damage was induced using carbon tetrachloride (CCl4) while silymarin served as a positive control.

Aim: This study is aimed at testing the hepatoprotective potentials of A. indica seed oil on Wistar rats.

Method: Hepatotoxicity was induced by the administration of 1.0 mL/kg of CCl4 subcutaneously to 72 healthy Wistar rats of both sexes (weight range: 145 g – 315 g). The seed oil of A. indica was orally administered daily in various doses of 0.25 mL/kg, 0.5 mL/kg and 1.0 mL/kg for 14 days. Animal body and organ weights were recorded, while blood and liver tissues were collected for biochemical, haematological and histological analyses.

Results: Treatment with neem seed oil lowered the aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase levels significantly (P < 0.05) in a dose-dependent manner compared to the control. The haematological parameters, organ weight index and animal body weight showed no significant difference (P > 0.05) when compared with the control. Histology assessment was in agreement with the biochemical result as tissues of CCl4 exhibited significant fatty tissue accumulation, as opposed to that of 0.25 mL/kg neem treatment, which showed only moderate accumulation of fatty tissues, while higher doses, 0.5 mL/kg and 1.0 mL/kg, showed a healthy liver as compared with the control.

Conclusion: The result of this study revealed that neem seed oil had a dose-dependent hepatoprotective effect on the experimental rats.


Azadirachta indica; Hepatoprotective; silymarin; carbon tetrachloride


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Crossref Citations

1. Effect of chronic administration of aqueous extract of Neem (Azadirachta indica) leaves on Paracetamol-induced hepatotoxicity in Wistar albino rats
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Current Perspectives on Medicinal and Aromatic Plants (CUPMAP)  year: 2022  
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