Original Research

Anti-diarrhoeal activity of aqueous extract of Cochlospermum planchonii (Hook Fx. Planch) leaves in female Wistar rats

Musa T. Yakubu, Omowunmi D. Amoniyan, Mariam O. Mohammed, Christine I. Assin, Jemilat O. Abubakar, Saoban S. Salimon, Sikemi A. Omar
Journal of Medicinal Plants for Economic Development | Vol 4, No 1 | a81 | DOI: https://doi.org/10.4102/jomped.v4i1.81 | © 2020 Musa T. Yakubu, Omowunmi D. Amoniyan, Mariam O. Mohammed, Christine I. Assin, Jemilat O. Abubakar, Saoban S. Salimon, Sikemi A. Omar | This work is licensed under CC Attribution 4.0
Submitted: 02 January 2020 | Published: 30 June 2020

About the author(s)

Musa T. Yakubu, Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, Ilorin, Nigeria
Omowunmi D. Amoniyan, Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, Ilorin, Nigeria
Mariam O. Mohammed, Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, Ilorin, Nigeria
Christine I. Assin, Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, Ilorin, Nigeria
Jemilat O. Abubakar, Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, Ilorin, Nigeria
Saoban S. Salimon, Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, Ilorin, Nigeria
Sikemi A. Omar, Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, Ilorin, Nigeria


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Abstract

Background: The folkloric use of Cochlospermum planchonii is yet to be substantiated with scientific evidence.

Aim: The aqueous extract of C. planchonii leaves was evaluated for anti-diarrhoeal activity at 125, 250 and 500 mg/kg body weight in female Wistar rats.

Setting: This research is a phytopharmacological investigation.

Methods: Animal were monitored for indicators of diarrhoea in the 3 models after treatments.

Results: An aqueous extract of C. planchonii leaves contained 10 secondary metabolites, with alkaloids (16.05 mg/L) occurring the most, whilst quinones (0.7 mg/L) were the least. The extract significantly (p < 0.05) prolonged the onset time of diarrhoea, decreased water content, fresh weight and total number of wet faeces in a dose-dependent manner, and increased the percentage inhibition of defecation. The extract produced dose-specific changes on intestinal superoxide dismutase, glucose and reduced glutathione whereas the levels of intestinal Na+/K+-ATPase, alkaline phosphatase, catalase, nitric oxide were significantly (p < 0.05) increased in the castor oil-induced diarrhoeal model. The masses and volumes of intestinal fluid decreased significantly (p < 0.05) whereas the inhibition of intestinal fluid content increased like those of atropine-treated diarrhoeal rats in the enteropooling model. The extract dose-dependently decreased the distance travelled by the charcoal meal and increased the intestinal nitric oxide and acetylcholinesterase in the charcoal meal transit model.

Conclusion: The aqueous extract of C. planchonii leaves exhibited anti-diarrhoeal activity via anti-motility and anti-secretory means. The flavonoids, alkaloids, tannins, phenolics and saponins might have acted to enhance the activities of Na+/K+-ATPase, antioxidant enzymes, intestinal glucose levels and the neurotransmitters.


Keywords

anti-diarrhoea; castor oil; Cochlospermum planchonii; Cochlospermacea; acetylcholine esterase

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