Original Research

Green tea (Camellia sinensis) aqueous extract improved human spermatozoa functions in vitro

Mmaphulane A. Setumo, Solomon SR Choma, Ralf Henkel, Chinyerum S. Opuwari
Journal of Medicinal Plants for Economic Development | Vol 6, No 1 | a166 | DOI: https://doi.org/10.4102/jomped.v6i1.166 | © 2022 Mmaphulane A. Setumo, Solomon SR Choma, Ralf Henkel, Chinyerum S. Opuwari | This work is licensed under CC Attribution 4.0
Submitted: 11 May 2022 | Published: 26 October 2022

About the author(s)

Mmaphulane A. Setumo, Department of Pathology, Faculty of Health Sciences, University of Limpopo, Polokwane, South Africa
Solomon SR Choma, Department of Pathology, Faculty of Health Sciences, University of Limpopo, Polokwane, South Africa
Ralf Henkel, Department of Medical Biosciences, Faculty of Natural Sciences, University of the Western Cape, Bellville, South Africa; Department of Metabolism, Digestion and Reproduction, Imperial College London, London, United Kingdom; and LogixX Pharma, Berkshire, United Kingdom
Chinyerum S. Opuwari, Department of Medical Biosciences, Faculty of Natural Sciences, University of the Western Cape, Bellville, South Africa


Share this article

Bookmark and Share

Abstract

Background: Idiopathic causes of infertility is associated with oxidative stress. Antioxidants are known to scavenge the excessive production of reactive oxygen species (ROS). Green tea (Camellia sinensis) contains polyphenols that enhance its antioxidant potential.

Aim: This study focused on the impact of aqueous green tea extract on normozoospermic human spermatozoa.

Setting: Department of Medical Biosciences, University of the Western Cape (UWC), South Africa.

Methods: Semen samples obtained using masturbation method following three to five days of sexual abstinence from consenting men (n = 59) at the University of the Western Cape (UWC) were liquefied and analysed. Normozoospermic samples were selected according to the World Health Organization (WHO) 5th guideline. Thereafter, semen samples (7.5 × 106 /mL) were washed in human tubular fluid (HTF; 10 min at 300 ×g) and exposed to aqueous extracts of green tea (0 μg/mL, 0.4 μg/mL, 4 μg/mL, 40 μg/mL, 405 μg/mL) for 1 h with various sperm parameters analyzed. Human tubular fluid supplemented with bovine serum albumin (HTF-BSA; 10%) served as control.

Results: Sperm motility, reactive oxygen species production, across some reaction and deoxyribonucleic acid (DNA) fragmentation decreased significantly, particularly at the highest concentration (405 μg/mL; p < 0.001). A substantial increase in the percentage of viable spermatozoa and those with intact mitochondrial membrane potential (MMP) were observed (p < 0.001).

Conclusion: Aqueous extract of green tea prolonged sperm viability and MMP while reducing sperm intracellular ROS production, capacitation and across some reaction and DNA fragmentation, and may be attributed to its antioxidant potential. However, a high concentration of the extract appears to be detrimental to the functioning of human spermatozoa.


Keywords

Camellia sinensis; green tea; human spermatozoa; antioxidants; infertility; medicinal plants; oxidative stress.

Metrics

Total abstract views: 201
Total article views: 246


Crossref Citations

No related citations found.